By Josie A. Beeley (auth.), Nicholas Catsimpoolas, James Drysdale (eds.)
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It was once approximately 1985 while either one of the authors begun their paintings utilizing multigrid equipment for strategy simulation difficulties. This occurred in established from one another, with a totally varied heritage and diversified intentions in brain. at present, a few very important monographs seemed or were in training.
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I974a, Protein composition of human submandibular secretions, Archs. Biochem. Biophys. 161:134. Mayo, J. , and Carlson, D. , 1974b, Isolation and properties offour a-amylase isozymes from human submandibular saliva, Archs. Biochem. Biophys. 163:498. , 1968, Hydrolysis of maltose by Taka-amylase A, J. Biochem. (Tokyo) 63:632. Oppenheim, F. , Hay, D. , 1971, Proline-rich proteins from human parotid saliva. r. Isolation and partial characterization, Biochemistry 10:4233. Reisner, A. , Nemes. , 1975, The use ofCoomassie Brilliant Blue G250 perchioric acid solution for staining in electrophoresis and isoelectric focusing on polyacrylamide gels, Anal.
Gels were stained with Coomassie Blue. NHP: nonheme protein. ) E. Identification of Animal Hemoglobins Structural studies of animal hemoglobins have been very useful in determining phylogenetic relationships between species and establishing a time table for evolutionary branch points (Dayhoff, 1972). Seal (1969) has used electrophoretic patterns to determine the relationships between 112 different carnivore species. This approach is complicated by the fact that there is considerable heterogeneity among hemoglobins of certain species.
Is a double heterozygote, inheriting the sickle gene from one parent and the ,8-thalassemia gene from the other. , ,B+-thalassemia). The increase in Hb F reflects compensation for the impaired production of ,8A-chains. c. Identification of Hemoglobin Variants Gel electrofocusing has been so useful in the identification of hemoglobin variants that it is now employed for routine diagnostic evaluation in many laboratories. In addition, the technique can be adopted to large 33 GEL ELECTROFOCUSING IN HEMOGWBIN ANALYSIS population surveys.